NK-CELL 3D-FACS-ANALYSIS-ADCC Russian patent published in 2016 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine, namely to biotechnology, and may be used to detect in vitro an antibody possessing an effector function. That is ensured by mixing killer cells and tumour cells, adding approximately 104 cells of each type per 200 mcl into wells of a multi-well plate. The multi-well plate is centrifuged to induce a formation of a three-dimensional spheroid. Each of the produced antibodies is placed into a separate well of the multi-well plate and incubated for a period of time making from approximately 20 to approximately 72 hours. The cells are analysed in the wells of the multi-well plate by means of a cell sorter with fluorescence excitation thereby assessing the effector function of the antibody. The group of inventions also refers to using the three-dimensional spheroid or aggregate, which contains tumour cells and natural killer cells, for the purpose of assessing the effector function of the antibody combination.

EFFECT: antibodies possessing the effector function are detected; dead to viable cell ratio has been disclosed exceeding 1.

12 cl, 7 dwg, 5 ex