FIELD: biotechnologies.
SUBSTANCE: invention relates to a set of sequences of primers and allele-specific probes for simultaneous gene diagnostics of two mutant alleles causing a complex of spine abnormalities (CVM) and deficit of leucocytal adhesion (BLAD) in cattle. The set includes sequences of primers and allele-specific probes, which have the following structure: CVM_up gattctcaagagcttaattctaagga, CVM_low aagtaaaccccagcaaagccac, CVM_Wt (FAM)-aggtctcatggcagttct-(BHQ1), CVM_m (R6G)-catggcatttctcacagcat-(BHQ2), BLAD_up ttaggcagttgcgttc, BLAD_low acgttgacgaggtcatccacca, BLAD_Wt (ROX)-accccatcgacctgtacta-(BHQ1), BLAD_m (Cy5)-ccatcggcctgtactacct-(BHQ2).
EFFECT: invention makes it possible to simultaneously diagnose CVM and BLAD in cattle by PCR method in real time, to reduce time of performance of a polymerase chain reaction in real time.
3 dwg, 2 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF SIMULTANEOUS GENODIAGNOSTIC OF TWO MUTANT ALLELES CAUSING CVM AND BLAD IN CATTLE, AND TEST SYSTEM FOR IT | 2015 |
|
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| METHOD FOR GENETIC DIAGNOSIS OF MUTANT ALLELE THAT CAUSES SHORT SPINE SYNDROME OR BRACHYSPINE SYNDROME IN CATTLE AND A TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2016 |
|
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| SET OF PRIMERS SEQUENCE AND ALLELE-SPECIFIC PROBES FOR SIMULTANEOUS FOUR MUTANT KAPPA-CASEIN ALLELES GENE DIAGNOSTICS IN BOVINE CATTLE | 2017 |
|
RU2646140C1 |
| METHOD FOR SIMULTANEOUS GENODIAGNOSTIC OF FOUR MUTANT ALLELES OF KAPPA-CASEIN IN CATTLE AND TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2017 |
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RU2691995C2 |
| TEST SYSTEM FOR DETECTING SINGLE NUCLEOTIDE SUBSTITUTION (A→G) OF POSITION 383 OF CD18 GENE ASSOCIATED WITH DEFICIT OF LYMPHOCYTE ADHESION IN COWS (BLAD), USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION | 2019 |
|
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| SET OF SEQUENCES OF PRIMERS AND ALLELE-SPECIFIC PROBES FOR SIMULTANEOUS GENODIAGNOSIS OF FOUR MUTANT ALLELES OF BETA-CASEIN IN CATTLE | 2022 |
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RU2798281C1 |
| METHOD FOR CARRYING OUT PCR WITH ALLELE-SPECIFIC PROBES FOR GENOTYPING CATTLE BY THE ALLELES A AND K OF THE DGAT1 GENE | 2018 |
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| METHOD FOR SIMULTANEOUS GENE DIAGNOSIS OF FOUR ALLELES OF BETA-CASEIN IN CATTLE AND TEST SYSTEM FOR ITS IMPLEMENTATION | 2022 |
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RU2815238C2 |
| METHOD FOR CATTLE GENOTYPING BY 1401G/T ALLELES lhcgr (ss52050737) GENE BY PCR IN REAL TIME | 2019 |
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RU2788734C2 |
