FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Proposed test system is a complex, which includes a specific set of reagents, procedure for preparing a reaction mixture and specially developed program for amplification of CD18 gene by polymerase chain reaction (PCR) "in real time". Specific oligonucleotide primers are used for PCR.
EFFECT: invention enables detecting carriers of the syndrome of insufficient adhesion of lymphocytes in cattle (BLAD) in populations of cattle of black-and-white roots (Holstein, black-and-white, Yaroslavl and other related breeds).
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