FIELD: biotechnologies.
SUBSTANCE: test system includes a mix of primers, complementary to the ends of different chains of a required DNA matrix fragment for PCR, restriction endonuclease with a buffer and a process mix for PCR with a Taq-polymerase. Being the mix of primers it contains a pair of deoxyoligonucleotides with the activity of direct and reverse primers in the reverse transcription reaction and polymerase chain reaction with the following structure: Nat2(F) MR1ACCWAAYCAGCCAGR1AAGC and Nat2(R) AGMCCSACCAWACAGYAMACC, where F and R - direct and reverse primers respectively, and A, G, S, R1, W, M, S and Y - code identifications of nucleotides.
EFFECT: invention allows finding with high reliability NAT2 gene expression of a human being.
6 dwg, 3 tbl, 2 ex
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RU2522801C2 |
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RU2694501C1 |
| SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2 | 2021 |
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| METHOD FOR DETERMINING THE LEVEL OF EXPRESSION OF THE GENE ENCODING IL-18 IN THE TISSUES OF THE EYE OF THE ORYCTOLAGUS CUNICULUS RABBIT, AND DETERMINATION KIT | 2020 |
|
RU2745323C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR THEREOF FOR INDICATING IMMUNODEFICIENCY VIRUSES AND LEUKAEMIA OF CATS IN CLINICAL MATERIAL BY MULTIPLEX POLYMERASE CHAIN REACTION | 2015 |
|
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| TEST SYSTEM FOR DETECTION OF THE VIRUS GENOME OF PARAINFLUENZA 3 TYPES AT CATTLE WITH A MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME MODE | 2018 |
|
RU2681473C1 |
| TEST SYSTEM OF DETECTING DNA OF AFRICAN SWINE FEVER VIRUS BY REAL-TIME POLYMERASE CHAIN REACTION | 2017 |
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RU2645263C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF RNA OF RABIES VIRUS AND METHOD OF IDENTIFICATION OF RNA OF RABIES VIRUS BY MEANS OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION (RT-PCR) | 2014 |
|
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| TEST SYSTEM FOR DETECTING CORONAVIRUS INFECTION PATHOGEN GENOME IN CATTLE BY MEANS OF MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME | 2018 |
|
RU2694499C1 |
