FIELD: biotechnologies.
SUBSTANCE: method includes performing of RT-PCR with oligonucleotide primers. The primers have the following nucleotide sequences: fp_850_gp_rabv 5'TTAGACTTATGGATGGAACATGGGT3', rp_850_gp_rabv 5'AGTGACTGACACCTCCCTCCCT3', fp_350_gp_rabv 5'TCAGACGAAATTGAGCACCTTGT3', rp_350_gp_rabv 5'ACCTCCCCCCAACTCTTAAACA3'. RT-PCR is performed in two rounds, and in case of a positive reaction the fragment corresponding to the size in the first round - 755 bps, in the second - to the size 259 bps, is synthesised.
EFFECT: invention allows to identify RNA strains and isolates of rabies virus of various origin at early stages of clinic implication, and also to reduce the cost of diagnostics.
2 cl, 2 dwg, 3 tbl, 4 ex
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Authors
Dates
2016-02-10—Published
2014-10-02—Filed