SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF RNA OF RABIES VIRUS AND METHOD OF IDENTIFICATION OF RNA OF RABIES VIRUS BY MEANS OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION (RT-PCR) Russian patent published in 2016 - IPC C12Q1/68 C12N15/11 

Abstract RU 2575088 C1

FIELD: biotechnologies.

SUBSTANCE: method includes performing of RT-PCR with oligonucleotide primers. The primers have the following nucleotide sequences: fp_850_gp_rabv 5'TTAGACTTATGGATGGAACATGGGT3', rp_850_gp_rabv 5'AGTGACTGACACCTCCCTCCCT3', fp_350_gp_rabv 5'TCAGACGAAATTGAGCACCTTGT3', rp_350_gp_rabv 5'ACCTCCCCCCAACTCTTAAACA3'. RT-PCR is performed in two rounds, and in case of a positive reaction the fragment corresponding to the size in the first round - 755 bps, in the second - to the size 259 bps, is synthesised.

EFFECT: invention allows to identify RNA strains and isolates of rabies virus of various origin at early stages of clinic implication, and also to reduce the cost of diagnostics.

2 cl, 2 dwg, 3 tbl, 4 ex

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RU 2 575 088 C1

Authors

Ivanov Arkadij Vasil'Evich

Khismatullina Nailja Anvarovna

Usol'Tsev Konstantin Valer'Evich

Guljukin Aleksej Mikhajlovich

Aleksandrova Natal'Ja Mikhajlovna

Juzhakov Anton Gennad'Evich

Sabirova Valentina Vasil'Evna

Chernov Al'Bert Nikolaevich

Ivanov Aleksandr Arkad'Evich

Zaberezhnyj Aleksej Dmitrievich

Samerkhanov Il'Nur Irshatovich

Parshikova Anna Vladimirovna

Faizov Tagir Khadievich

Dates

2016-02-10Published

2014-10-02Filed