FIELD: medicine.
SUBSTANCE: invention relates to medicine and can be used for pathomorphological diagnosis of chronic appendectomy. Method is carried out as follows: tissue sample is fixed, sections are prepared, incubated with a reagent and treated with a developing agent, dehydrated and enclosed in medium. Reagent used is antibody p44 MAPK (Erkl) (MARK3) diluted in ratio of 1:100-1:250, duration of incubation with reagent in a moist chamber at 25 °C is 60 minutes.
EFFECT: method enables to identify specific colour in product in subserous and muscular layers of wall of appendix to diagnose chronic appendicitis.
1 cl, 1 ex, 4 dwg
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