METHOD FOR DIAGNOSIS FOR IN VITRO OF SPECIFIC BODY SENSITISATION TO BACTERIAL ALLERGENS Russian patent published in 2016 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: method consists in selection and preparation of venous blood, introduction of test preparation containing bacterial antigens, and comparison of values of luminol-dependent chemoluminescence (CL) with similar values received for a healthy body. Into collected patient's blood anticoagulant is introduced and samples containing (0.95-1.05)×10⁶ leukocytes are formed; the samples, with the help of Hanks solution, are enlarged to the volume of 0.69 ml and blood samples are obtained. Tested preparation of bacterial antigens or a mixture of the tested preparation of bacterial antigens with antiallergic tavegil and a control preparation in the amount of 0.01 ml is introduced into the obtained blood samples and incubated during constant stirring at a temperature of 37 °C for 45 minutes. Then into the incubated samples there is introduced a luminol activator in the amount of 2 mM and use is made of a chemiluminometer to measure the level of spontaneous CL for the said activator; after that, into the same samples a glow stimulator is introduced -barium sulphate in the amount of 2 mg/ml; the level of stimulated CL for the said activator is also registered; at that, the measurement of CL is performed during constant stirring at a temperature of 37 °C for 45 minutes to obtain time dependencies of CL level. HIV protease inhibitor indices are calculated by expression PI = Sba/Sc, where Sba; Sc is the area under the curves of time dependence of spontaneous and stimulated CL for the tested and control preparations, respectively, by which specific sensitisation to bacterial antigens is diagnosed.

EFFECT: disclosed is a differential diagnostic technique for in vitro specific patient's sensitisation to bacterial allergens.

1 cl, 2 tbl, 2 ex