FIELD: medicine, laboratory diagnostics.
SUBSTANCE: one should isolate neutrophilic leukocytes, form immune complexes, measure background luminescence of incubation medium (A), add neutrophils, measure the level of spontaneous chemiluminescence (B), introduce immune complex and detect the value of stimulated chemiluminescence (C), calculate the coefficient of stimulation (CS) and at CS≥0.3 it is possible to diagnose allergic reaction. The innovation provides higher accuracy and specificity in detecting the presence of allergic sensitization to certain antigen, the chance for screening serial assay, excludes additional body sensitization associated with introducing allergens in case of skin sample.
EFFECT: higher accuracy of allergodiagnostics.
1 cl, 5 ex, 6 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DIAGNOSING STAPHYLOCOCCAL ALLERGY IN CASE OF ALLERGIC RHINITIS | 2013 |
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| METHOD FOR DIAGNOSIS FOR IN VITRO OF SPECIFIC BODY SENSITISATION TO BACTERIAL ALLERGENS | 2014 |
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| METHOD OF DIAGNOSING SPECIFIC ALLERGEN SENSITIZATION OF BODIES OF WORKERS IN HYDROLYTIC-YEAST PRODUCTION PLANTS | 1994 |
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| METHOD OF DETECTION OF SENSIBILIZATION TO MEDICINAL ALLERGENS | 1995 |
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| METHOD OF LIFE-TIME DIAGNOSIS OF CATTLE TUBERCULOSIS | 2020 |
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| in vitro DIAGNOSTIC TECHNIQUE FOR PSEUDOALLERGEN HYPERSENSITIVITY AND SELECTING ANTIALLERGIC MEDICINES | 2014 |
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| METHOD OF LIFETIME DIFFERENTIAL DIAGNOSIS OF BOVINE TUBERCULOSIS AND MYCOBACTERIOSES | 2018 |
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RU2687553C1 |
| METHOD FOR DETERMINING SENSITIZATION TO ALLERGENS | 1998 |
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| METHOD OF ESTIMATION OF SENSITIZATION TO CHEMICAL COMPOUNDS | 2000 |
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RU2180117C1 |
