BIOLOGICALLY-ACTIVE RADIOLABELED Cry1Fa AND RECEPTOR BINDING ASSAY METHODS Russian patent published in 2016 - IPC C07K14/325 C12Q1/16 

Abstract RU 2601161 C2

FIELD: biotechnology.

SUBSTANCE: present invention relates to biotechnology and can be used in agriculture. Obtained biologically active radiolabeled Cry1Fa protein can be used in competitive binding assays with other Cry toxins for determining activity of tested compounds.

EFFECT: invention enables to obtain a cysteine-specific radiolabeled Cry1Fa protein which retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner.

6 cl, 2 dwg, 1 tbl, 9 ex

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RU 2 601 161 C2

Authors

Evans Stiven L.

Li Tszyantsyuan

Shits Dzhoel Dzh.

Dates

2016-10-27Published

2011-12-16Filed