FIELD: biotechnologies.
SUBSTANCE: group of inventions relates to biotechnology. Disclosed are versions a multimeric protein structure, which hydrolyses terminal α-galactosyl moiety of globotriaosylceramide and comprises two α-galactosidase monomers covalently bonded to each other through binding moiety, where multimeric protein structure is a dimeric protein structure. Disclosed are methods for preparing said versions of multimeric protein structures, involving reaction of α-galactosidase with a cross-linking agent. Disclosed is a pharmaceutical composition containing said multimeric protein structure.
EFFECT: group of inventions enables to obtain multimeric protein structures with high stability and high activity in vivo compared to native form of α-galactosidase, which can be used in treating Fabry disease.
25 cl, 83 dwg, 10 tbl, 19 ex
| Title | Year | Author | Number |
|---|---|---|---|
| TREATMENT SCHEME FOR TREATMENT OF FABRY'S DISEASE, USING STABILIZED α-GALACTOSIDASE | 2018 |
|
RU2793184C2 |
| TREATMENT OF α-GALACTOSIDASE A INSUFFICIENCY | 2000 |
|
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| PRODUCTION OF HIGH-MANNOSE PROTEINS IN PLANT CULTURES | 2009 |
|
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| PRODUCTION OF ACTIVE HIGHLY PHOSPHORYLATED N-ACETYLGALACTOSAMINE-6-SULPHATASE AND USE THEREOF | 2011 |
|
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| METHODS AND COMPOSITIONS FOR TREATING FABRY DISEASE | 2020 |
|
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| PRODUCING ACTIVE HIGHLY PHOSPHORYLATED HUMAN LYSOSOMAL SULPHATASE ENZYMES AND USE THEREOF | 2009 |
|
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| PRODUCTION OF HIGH-MANNOSE PROTEINS IN VEGETABLE CULTURES | 2004 |
|
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| PROTEIN COMPLEX, OBTAINED BY USING FRAGMENT OF IMMUNOGLOBULIN, AND METHOD OF SUCH COMPLEX OBTAINING | 2004 |
|
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| IMMUNOGENIC COMPOSITIONS OF MULTIPLE ANTIGEN PRESENTATION, METHODS AND APPLICATIONS RELATING THERETO | 2012 |
|
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| N-GLYCANES CONTAINING GALACTOSE-ALPHA-1,3-GALACTOSE IN GLYCOPROTEIN PRODUCTS PRODUCED FROM CHO CELLS | 2009 |
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