N-GLYCANES CONTAINING GALACTOSE-ALPHA-1,3-GALACTOSE IN GLYCOPROTEIN PRODUCTS PRODUCED FROM CHO CELLS Russian patent published in 2013 - IPC C12Q1/34 C12Q1/54 C12N5/07 C12N5/10 

FIELD: biotechnologies.

SUBSTANCE: multiple populations of Chinese hamster ovary (CHO) cells undergo screening for ability to produce glycoproteins, which include glycans, containing terminal remains of galactose-alpha-1-3-galactose. The method of screening includes assessment of the level of glycans containing terminal remains of galactose-alpha-1-3-galactose, in multiple populations of Chinese hamster ovary (CHO) cells and selection of populations producing the specified glycans at the target level. Glycoprotein compositions produced by the Chinese hamster ovary (CHO) cells are analysed for availability of the specified glycans. The method of assessment of the glycoprotein composition includes measurement of the amount of terminal galactose-alpha-1-3-galactose.

EFFECT: using the invention makes it possible to track and control content of terminal remains of galactose-alpha-1-3-galactose while using CHO cells as producers of therapeutic glycoprotein products.

28 cl, 11 dwg, 2 tbl, 1 ex