METHOD FOR PRODUCTION OF PROTEOLYTIC PREPARATION FOR MEDICAL USE Russian patent published in 2017 - IPC C12N9/50 C12N9/64 A61K38/48 

FIELD: medicine, pharmacy.

SUBSTANCE: invention refers to biotechnology. A method for extraction of proteolytic enzymes from the digestive organs of fish is proposed. The method includes tissue homogenizing in a buffer, selection of enzymes in the presence of ammonium sulfate, at that, nucleic acids and lipid-protein complexes are removed from the extract prior to protein precipitation in ammonium sulfate by treatment with a polyethyleneimine solution, added to a final concentration from 0.1 to 0.01 by weight %. Residual pyrogens are separated on a hydrophobic sorbent Sferotsell C-80 with further repeated desalination of the extract. The enzyme solution is sterilized by filtration through a membrane with pore size of 0.2 microns, followed by production of lyophilized formulation of the enzyme preparation.

EFFECT: invention allows to obtain proteolytic enzymes with increased collagenolytic activity.

6 cl, 1 dwg, 1 tbl, 7 ex