METHOD OF PREPARING HUMAN ALPHA-INTERFERON Russian patent published in 1995 - IPC

FIELD: biotechnology. SUBSTANCE: method involves bacterial mass disruption of producer Pseudomonas putida 84 containing recombinant plasmid pVG-3. Fractionation in lysate buffer is carried out at pH 4.5-5, and concentration and diafiltration - through filter casette type PTGC at limit 10000 dalton. Chromatography is performed on cellulose DE-52 using 0.04-0.06M acetate buffer (pH 4.8-5.2) for elution, then on cellulose CM-52 using linear gradient of 0.1M acetate buffer and 0.1M acetate buffer with 0.3-0.5M sodium chloride (pH 4.8-5.2), and gel-filtration on Sephadex G-100 equilibrated with 0.05-0.15M phosphate buffer with 0.15-0.25M sodium chloride (pH 7.15-7.25). EFFECT: increased purification degree, decreased cost of the end product.