METHOD FOR DETERMINING TOXICITY OF CHEMICALS GENERATING ACTIVE OXYGEN FORMS Russian patent published in 2017 - IPC C12Q1/66 C12N1/21 C12N15/52 

Abstract RU 2614267 C1

FIELD: chemistry.

SUBSTANCE: method provides adding riboflavin to the final concentration of 1⋅10-5 mg/ml - 1⋅10-3 mg/ml into the Escherichia coli K12 MG1655 culture with the pColD-lux plasmid, in which the bioluminescence lux operon of marine photobacteria Photobacterium leiognathi, Vibrio fischeri or Photorabdus luminescens is placed under the control of the pColD promoter. Adding the test substance and determining the luminescence intensity of the resulting suspension and the control are carried out. The damaging effect of the test substance is estimated by the suspension luminescence intensity deviation from the control.

EFFECT: increasing the induction factor.

4 cl, 1 dwg, 2 ex

Similar patents RU2614267C1

Title Year Author Number
METHOD FOR DETERMINING CHEMICALS GENOTOXICITY 2016
  • Chistyakov Vladimir Anatolevich
  • Prazdnova Evgeniya Valerevna
  • Chmykhalo Viktor Konstantinovich
  • Bren Anzhelika Borisovna
  • Belik Timur Viktorovich
  • Mazanko Mariya Sergeevna
RU2614122C1
SET OF lux-BIOSENSORS FOR DETERMINING GENOTOXIC PRODUCTS OF INCOMPLETE OXIDATION OF UNSYMMETRICAL DIMETHYL HYDRAZINE IN ENVIRONMENT 2014
  • Manukhov Il'Ja Vladimirovich
  • Gorbunov Mikhail Alekseevich
  • Degtev Dmitrij Il'Ich
  • Zavil'Gel'Skij Gennadij Borisovich
  • Kessenikh Andrej Grigor'Evich
  • Konopleva Marija Nikolaevna
  • Kotova Vera Jur'Evna
  • Krasnopeeva Ekaterina Dmitrievna
  • Motovilov Konstantin Aleksandrovich
  • Osetrova Marija Stanislavovna
  • Chalkin Stanislav Filippovich
RU2569156C1
LUX-BIOSENSORS SET FOR DETECTION OF TOXIC PRODUCTS OF INCOMPLETE OXIDATION OF NON-SYMMETRIC DIMETHYLHYDRAZINE IN MEDIUM 2015
  • Kessenikh Andrej Grigorevich
  • Manukhov Ilya Vladimirovich
  • Vagapova Elmira Ramilevna
  • Vysokikh Mikhail Yurevich
  • Konopleva Mariya Nikolaevna
  • Kotova Vera Yurevna
  • Gorbunov Mikhail Alekseevich
  • Chalkin Stanislav Filippovich
  • Zavilgelskij Gennadij Borisovich
RU2626569C2
KIT OF LUX-BIOSENSORS FOR HEPTYL DETECTION IN MEDIUM 2005
  • Zavil'Gel'Skij Gennadij Borisovich
  • Kotova Vera Jur'Evna
  • Manukhov Il'Ja Vladimirovich
  • Kondrat'Ev Andrej Dmitrievich
  • Sambros Vitalij Vasil'Evich
  • Shatrov Jakov Timofeevich
  • Chalkin Stanislav Filippovich
RU2297450C2
SET OF lux-BIOSENSORS FOR DETECTING HYDROPHOBIC DETERGENTS IN MEDIUM 2007
  • Zavil'Gel'Skij Gennadij Borisovich
  • Kotova Vera Jur'Evna
  • Manukhov Il'Ja Vladimirovich
  • Mel'Kina Ol'Ga Evgen'Evna
RU2355760C1
METHOD FOR IDENTIFYING PROMISING PROBIOTIC STRAINS FROM NATURAL MEDIA 2021
  • Bren Anzhelika Borisovna
  • Mazanko Mariia Sergeevna
  • Prazdnova Evgeniia Valerevna
  • Ermakov Aleksei Mikhailovich
  • Popov Igor Vitalevich
  • Chistiakov Vladimir Anatolevich
  • Chikindas Mikhail Leonidovich
RU2772351C1
METHOD OF BIODETECTION OF POLYAROMATIC HYDROCARBONS 2000
  • Chistjakov V.A.
  • Vojnova N.V.
  • Timoshkina N.N.
RU2198929C2
METHOD TO DETECT GENOTOXICITY OF CHEMICAL SUBSTANCES 2000
  • Sazykina M.A.
  • Chistjakov V.A.
  • Vojnova N.V.
RU2179581C1
SET OF STRESS-INDUCIBLE LUX BIOSENSORS BASED ON BACILLUS SUBTILIS CELLS FOR STUDYING TOXICITY MECHANISMS 2022
  • Bazhenov Sergei Vladimirovich
  • Kessenikh Andrei Grigorevich
  • Novoiatlova Uliana Sergeevna
  • Gnuchikh Evgenii Iurevich
  • Kotova Vera Iurevna
  • Manukhov Ilia Vladimirovich
RU2811895C2
BIOCHEMILUMINESCENT METHOD FOR EVALUATION OF PHAGOCYTIC ACTIVITY OF NEUTROPHILS 2007
  • Derjabin Dmitrij Gennad'Evich
  • Karimov Il'Shat Fajzelgajanovich
RU2366953C2

RU 2 614 267 C1

Authors

Chistyakov Vladimir Anatolevich

Prazdnova Evgeniya Valerevna

Mazanko Mariya Sergeevna

Belik Timur Viktorovich

Chmykhalo Viktor Konstantinovich

Bren Anzhelika Borisovna

Dates

2017-03-24Published

2016-01-12Filed