FIELD: medicine.
SUBSTANCE: method comprises surgical material taking, plaque grinding and enzymatic treatment, filtration, cell suspension staining to determine live/dead cells, followed by staining with monoclonal antibodies, flow cytometry conducting, for cell suspension preparation, a nylon filter is used with a pore size of 40 microns.
EFFECT: increased separation efficiency and accuracy of determination of the live cells population required for the study of composition of the immune cells from atherosclerotic plaques at different models of the flow cytometer.
1 dwg, 2 tbl, 1 ex
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