DERIVATIVES OF BLOOD COAGULATION FACTORS VII AND VIIA, CONJUGATES AND COMPLEXES CONTAINING THEM AND THEIR APPLICATION Russian patent published in 2017 - IPC C07K19/00 C07K14/745 C07K7/00 C12N9/02 C07K17/14 C07K14/735 C12N15/63 C07K1/08 A61K38/08 A61K38/36 A61K38/44 A61K47/30 A61K47/50 A61K47/42 A61P7/04 A61K47/60 A61K47/65 

FIELD: biotechnology.

SUBSTANCE: invention relates to production of blood coagulation derivatives VII and VIIa, their conjugates with polymers capable of increasing the half-life of blood from the bloodstream, complexes containing them obtained by carrier binding to the conjugate, to genes encoding the derivatives, to expression vectors containing these genes, transformants with introduced expression vectors, to methods for their preparation, pharmaceutical compositions and treatment methods, and can be used in medicine to prevent or treat hemophilia or to improve blood coagulation. A FVII derivative is obtained which is linked at its C-terminus to the peptide linker for binding to a non-peptidyl polymer capable of increasing the half-life of FVII or FVIIa from the blood stream. At that, the peptide linker is a partial sequence of superoxide dismutase SOD1, its mutated sequence or GGGGSC sequence.

EFFECT: invention allows to obtain an FVII derivative capable of binding to a carrier increasing the half-life from the blood stream while maintaining FVII activity.

42 cl, 8 dwg, 3 tbl, 8 ex