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2 620 074

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IPC

C12Q1/18(2006-01-01)

C12Q1/68(2006-01-01)

C12N15/70(2006-01-01)

C12N1/21(2006-01-01)

C12R1/19(2006-01-01)

(21) (22)

Application

2016130354, 2016-07-25

(24)

Start date

2016-07-25

(22)

Actual filing date

2016-07-25

(45)

Published

2017-05-22

(72)

Inventor

Osterman Ilya AndreevichAndreyanova Ekaterina SergeevnaSergiev Petr VladimirovichEvfratov Sergej AleksandrovichDontsova Olga AnatolevnaIvanenkov Yan AndreevichLaptev Ivan GeorgievichPletnev Filipp IgorevichMarusich Elena IvanovnaLeonov Sergej ViktorovichGolovina Anna Yankovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Gosudarstvennyj Universitet Imeni M.V. Lomonosova"

RECOMBINANT PLASMID pDUALREP2 DNA AND STRAIN TRANSFORMED THEREBY FOR DETECTION OF SUBSTANCES AND MIXTURES, INHIBITING PROTEINS BIOSYNTHESIS AND/OR SOS-RESPONSE INDUCTION IN BACTERIA Russian patent published in 2017 - IPC C12Q1/18 C12Q1/68 C12N15/70 C12N1/21 C12R1/19

Abstract RU 2620074 C1

FIELD: biotechnology.

SUBSTANCE: genetic structure contains genes of two fluorescent proteins with distinct fluorescence spectra. Expression of one fluorescent protein gene is activated by stopped translation of the preceding genetically modified Escherichia coli tryptophan operon attenuator. Expression of the second gene is activated by de-repression of the preceding Escherichia coli sulA gene promoter. The genetic structure is a plasmid, transforming E. coli bacterial cells to obtain the E. coli tolC Δreporter strain.

EFFECT: obtained strain allows efficient analysis of a large number of substances or mixtures to find antibacterial agents, while identifying those that inhibit protein biosynthesis or induce SOS-response to DNA damage.

5 cl, 5 dwg, 1 tbl, 3 ex

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RU 2 620 074 C1

Authors

Osterman Ilya Andreevich

Andreyanova Ekaterina Sergeevna

Sergiev Petr Vladimirovich

Evfratov Sergej Aleksandrovich

Dontsova Olga Anatolevna

Ivanenkov Yan Andreevich

Laptev Ivan Georgievich

Pletnev Filipp Igorevich

Marusich Elena Ivanovna

Leonov Sergej Viktorovich

Golovina Anna Yankovna

Dates

2017-05-22—Published

2016-07-25—Filed