FIELD: medicine.
SUBSTANCE: there is designed recombinant plasmid DNA pYfi-gfp enabling production of chimeric fluorescent protein GFPaav with five modified amino acids, corresponding to the first five amino acids of protein YfiA with controlling a promotor of gene YfiA in presence of toxic agents. Plasmid has size 3730 base pairs. Recombinant strain Escherichia coli JM109-pYfi contains a genetic design under given invention and produces fluorescent protein GFPaav in presence of toxic agents damaging a cell.
EFFECT: invention allows registering the presence of toxic agents in a medium with increasing the fluorescence level of cells of produced strain.
2 cl, 4 dwg, 4 ex
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