CELL-FREE PROTEIN SYNTHESIS SYSTEM BASED ON STAPHYLOCOCCUS AUREUS CELLS, A METHOD OF PROTEIN SYNTHESIS BASED ON STAPHYLOCOCCUS AUREUS CELLS USING A CELL-FREE PROTEIN SYNTHESIS SYSTEM BASED ON STAPHYLOCOCCUS AUREUS CELLS AND A METHOD OF DETECTING PROTEIN SYNTHESIS INHIBITORS USING IT Russian patent published in 2023 - IPC C07K1/107 C12P21/02 C12N15/00 C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: proposed cell-free protein synthesis system based on Staphylococcus aureus cells, obtained by mixing the components of the reaction mixture in the specified order and volume ratio (vol.%); a method of protein synthesis in a cell-free system based on Staphylococcus aureus cells, including incubation of the claimed cell-free system at 37°C; a method of detecting inhibitors of protein synthesis based on Staphylococcus aureus cells, including incubation at 37°C potential inhibitors of protein synthesis in conjunction with a cell-free protein synthesis system based on Staphylococcus aureus cells, quantitative measurement of the synthesized protein by a luminescent or fluorescent radiation signal and determination of the ability to inhibit protein synthesis by the incidence of a light signal.

EFFECT: inventions expand the arsenal of agents for protein synthesis in open systems outside cells based on cellular metabolism, for screening antimicrobial substances in biotechnological and pharmaceutical industries.

6 cl, 2 dwg, 3 ex