FIELD: medicine.
SUBSTANCE: determining the level of expression of YKL-39 using TaqMan technology using specific primers and Sense probe 5'-aacaacaaggttatcatcaaggac-3', AntiSense 5'-tttgggattcttggttttgag-3', Probe FAM-5'-agtgaagtgatgctctaccagaccat-3'-BHQ1, PCR analysis of the expression of the YKL-39 protein gene, the relative expression of the YKL-39 protein gene was evaluated using the Pfaffl method in conventional units (CU) relative to the GAPDH referee gene. As the calibrator, normal breast tissue is used, in which, in the same manner as described above, the level of expression of the YKL-39 protein gene is 1 CU. With an expression level of YKL-39, more than 1 CU is predicted to be high, and at a YKL-39 protein expression level less than 1 CU, a low probability of a prolonged period of non-metastatic survival.
EFFECT: higher prediction accuracy.
1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREDICTING FIVE-YEAR NONMETASTATIC SURVIVAL IN BREAST CANCER PATIENTS BASED ON THE EXPRESSION OF THE YKL-39 AND CCL18 PROTEIN GENES | 2017 |
|
RU2659676C1 |
| METHOD FOR PREDICTING RISK OF LYMPHATIC CANCER SPREAD IN BREAST CANCER BASED ON YKL-39 PROTEIN GENE EXPRESSION | 2016 |
|
RU2632115C1 |
| METHOD FOR PREDICTING NON-METASTATIC SURVIVAL IN PATIENTS WITH BREAST CANCER ON THE BASIS OF EXPRESSION OF SOMATIC-STEM TRANSITION GENES IN RESIDUAL TUMOR AFTER PRE-OPERATIVE TREATMENT | 2018 |
|
RU2682879C1 |
| METHOD FOR PREDICTION OF DURATION OF NON-METASTATIC PERIOD IN PATIENTS WITH INVASIVE BREAST CARCINOMA OF NON-SPECIFIC TYPE | 2024 |
|
RU2838685C1 |
| METHOD FOR PREDICTING THE DEGREE OF RISK OF DEVELOPING HEMATOGENOUS METASTASES IN INVASIVE CARCINOMA OF A NON-SPECIFIC TYPE OF MAMMARY GLAND BASED ON THE ASSESSMENT OF THE EXPRESSION OF LIMCH1 PROTEIN | 2020 |
|
RU2755242C1 |
| METHOD FOR PREDICTING THE OUTCOME OF A DISEASE IN PATIENTS WITH LUMINAL AND THRICE NEGATIVE BREAST CANCER IN PATIENTS WHO DID NOT RECEIVE CHEMOTHERAPY OR HORMONE THERAPY IN THE NEOADJUVANT REGIMEN | 2017 |
|
RU2648523C1 |
| METHOD FOR PREDICTING DEVELOPMENT OF METASTASES IN PATIENTS WITH BREAST CANCER | 2018 |
|
RU2675236C1 |
| METHOD FOR PREDICTION OF RISK OF DEVELOPING DISTANT METASTASES IN PATIENTS WITH OPERABLE FORMS OF BREAST CANCER WITH METASTASES IN REGIONAL LYMPH NODES | 2020 |
|
RU2733697C1 |
| METHOD FOR PREDICTION OF RISK OF TUMOUR PROGRESSION IN PATIENTS WITH BREAST CANCER | 2024 |
|
RU2838618C1 |
| METHOD FOR PERSONALIZED ASSIGNMENT OF NEOAJUVANT CHEMOTHERAPY TO PATIENTS SUFFERING FROM LUMINAL TYPE B BREAST CANCER | 2015 |
|
RU2594251C1 |
