METHOD FOR ARACHIDONIC ACID PRODUCTION Russian patent published in 2017 - IPC A61K31/20 A61K35/407 C11C1/02 C07C57/03 C07C51/09 

FIELD: pharmacology.

SUBSTANCE: method for preparation of arachidonic acid from an animal raw material includes alkaline hydrolysis of lipids isolated from the raw material, followed by hydrolyzate acidification, iodine-lactonization reaction, iodine-lactone extraction from the reaction mixture with an organic solvent, iodine-lactone dilution with a silicone agent, neutralisation of the released iodine with sodium thiosulfate, fatty acids extraction with an organic solvent, where, prior to the iodine-lactonization reaction, lipid hydrolyzate is treated with lithium alkali in acetone and saturated acids are isolated as a precipitate, Iodine-lactonization is carried out by transferring lithium salts of unsaturated fatty acids to sodium salts, which are treated with iodine solution to form iodine-lactones of fatty acids at a ratio of the acids sum (arachidonic, eicosapentaenoic and docosahexaenoic):iodine equal to 1.0:1.0-1.2 mol/mol, at mixture temperature of 18-22°C, with duration of iodine solution addition to the reaction mixture of 30-60 minutes, followed by solutions maintaining for 60 minutes; after iodine-lactones extraction from the reaction mixture with an organic solvent, delta- and gamma-iodo-lactone separation is further carried out by chromatography on silica gel using benzene as the eluent; and the disclosure of delta-iodine-lactones of arachidonic and eicosapentaenoic acids is carried out with silicide at a ratio of arachidonic acid iodine-lactone + eicosapentaenoic acid/silicide = 1.0:1.0-1.2 mol/mol, mixture temperature of 18-22°C, reaction time of 120-180 minutes; after fatty acids extraction with an organic solvent, they are esterified with ethyl alcohol and arachidonic acid is isolated as ethyl ether by reverse phase high performance liquid chromatography (C-18) using an ethanol-water eluting system of 8.0:1.0-2.5 vol./vol.

EFFECT: easy and reproducible way to separate pure arachidonic acid from animal tissues, available for sale in laboratories and industrial plants using arachidonic acid in biochemical, medical and dietetic studies.

4 cl, 2 dwg, 1 tbl, 5 ex