METHOD FOR PRODUCTION OF HYPERIMMUNE SERUM CONTAINING HETEROLOGIC IMMUNOGLOBULINES AGAINST EBOLA FEVER Russian patent published in 2017 - IPC A61K39/42 C07K16/08 

FIELD: pharmacology.

SUBSTANCE: method for hyperimmune serum production contains heterologous immunoglobulins against Ebola fever, involves 4-fold immunization of horses with viral preparations that do not contain live Ebola virus, followedantigenic by blood collection from producer animals and hyperimmune serum isolation. As a viral antigenic preparation, a DNA preparation containing the Ebola virus glycoprotein gene and a HPV preparation containing virus-like particles comprising the Ebola virus glycoprotein gene are used, the 3-fold administration of the DNA preparation is performed intramuscularly according to the following scheme: 1st immunization - DNA preparation administration at a dose of 4 mg/animal on day 0, 2nd immunization - DNA preparation administration at a dose of 4 mg/animal on day 21-28 after the first administration of the drug, 3rd immunization - DNA preparation administration at a dose of 4 mg animal on day 21-28 after the second administration of the drug, the fourth immunization performed subcutaneously by HPV preparation administration at a dose× of 3109 HPV/animal on day 56 after the third administration of the DNA preparation, allowing to induce formation of common specific antibodies in titres of at least 1:75,000 and formation of neutralising antibodies to Ebola virus in titres of at least 1:640, and blood sampling for production of a hyperimmune serum containing immunoglobulins is carried out on day 10 -13 after immunization with the HPV preparation. The total time of immunization (from the 1st immunization to blood sampling) is 122 days.

EFFECT: simplification of technology for hyperimmune serum obtaining and reduced cost of its production.

3 cl, 4 dwg