METHOD FOR PURIFICATION OF SOIL POLLUTED BY OIL AND OIL PRODUCTS USING BIOPREPARATION Russian patent published in 2017 - IPC B09C1/00 

FIELD: biotechnology.

SUBSTANCE: method includes full mineral fertiliser introduction into the soil, soil moisture maintaining, periodic loosening or plowing, preparation of a ready to use aqueous dispersion from a dry biopreparation containing a water soluble protective medium and microorganisms - petroleum hydrocarbon destructors, including bacteria of epy Rhodococcus genus, and its application to the polluted soil, a biopreparation is used containing Rhodococcus strain of the Rhodococcus jialingie 1kp bacterium as a bacterium, as well as additional Lysinibacillus xylanilyticus 5 rb bacterium strain and Yarrowia lipolytica 2kp yeast strain, in which dried microorganisms are taken at a weight ratio of Rhodococcus jialingie 1kp : Lysinibacillus xylanilyticus 5 rb : Yarrowia lipolytica 2kp = (1-2):(1-1.5):(2-3). The protective medium of the biopreparation includes dehydrated yeast autolysate, glucose and nutritive mineral salts and trace elements containing at least nitrogen, phosphorus, potassium, magnesium, manganese, zinc and iron compounds, which are taken in the mass ratio of dehydrated yeast autolysate : glucose : salts and trace elements = (1-2):(0.5-1):( 1-2). Preparation of the ready-to-use aqueous dispersion of the biopreparation performed by dispersion of the calculated amount of dry biopreparation M (g) in g in the calculated volume V₁ (l) in 1 l of 0.4-0.6% aqueous solution of the full mineral fertiliser to obtain an initial dispersion containing the viable microorganisms of the preparation in an amount of 2×10⁹-4×10¹¹ colony forming units (CFU)/l, air blowing through it for 12-14 hours, followed by introduction of a diesel fuel or soil contaminant into the dispersion in an amount of 0.05-0.20% of the initial dispersion, re-purging through the air dispersion during 12-14 hours and dilution of the obtained concentrated dispersion 20-40 times with a calculated volume of water V₂ (l) in l. The ready-to-use dispersion is applied in an amount of 10-20 l/m² soil, soil moisture is maintained at a level of 60-70% and the first soil loosening or plowing is performed after application of the ready-to-use biopreparation dispersion and application of mineral fertiliser to the soil, and the numerical values of V₁ (n), M (r), and V₂ (n) are calculated by the formulas V₁ (n)=P (l/m²)×S (m²)/N, where P (l/m²) is the amount of ready-to-use biopreparation dispersion in l applied per 1 m² of polluted soil, S (m²) is the total area of the soil to be cleaned in m² and N is the degree of dilution of the concentrated dispersion with water, M (g)=K (CFU/l)×V₁ (l)/A (CFU/g), where K (CFU/l) is CFU content in 1 l of initial dispersion, A (CFU/g) is the known content of viable microorganisms of the biopreparation in CFU in 1 g of dry biopreparation and V₂ (n)=(N-1)×V₁ (l).

EFFECT: speed of soil purification from hydrocarbon pollution is increased by 5 times.

1 tbl, 13 ex