REPROGRAMMING OF EFFECTIVE PROTEIN INTERACTIONS FOR EPIGENETIC DEFECTS CORRECTION IN MALIGNANT TUMOUR Russian patent published in 2017 - IPC G01N33/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to a method for identification of candidate compounds that reprogram the histone-tagging protein from binding its related histone tag to binding to an unrelated histone tail tag, comprising: (a) calculated obtaining of a structural model of the active site of the transcribing protein in combination with an unrelated target histone tail tag, where the active site is modeled with a site for binding compounds candidate; (b) obtaining of a probe structure selected for non-competitive binding to the histone tail tag at the site to bind candidate compounds so as to form a stable triple reprogramming complex with the histone tail tag target and the active site of the transcribing; and (c) candidate compounds screening to identify those that, together with residues in the active site and the target tag of the histone tail, substantially reproduce one or more functional feature involved in the formation of a stable triple reprogramming complex.

EFFECT: improved method.

16 cl, 12 dwg, 5 tbl, 12 ex