METHOD FOR DETECTING NERVE FIBERS OF ADRENERGIC AND CHOLINERGIC NATURE IN TISSUE Russian patent published in 2005 - IPC

FIELD: medicine.

SUBSTANCE: method involves fixing material and setting histochemical reaction on detecting cholinergic nerve structures. The fixed material under study is incubated during 60-90 min in thermostat at 37°C in mixture containing acetylthiocholine iodide as substrate, washed, treated with alcohols in growing concentrations, cleared and enclosed into balsam. The material is fixed with 2% glyoxylic acid prepared on 0.1 M phosphate buffer solution having pH of 7,0. Histochemical reaction is set concurrently with material fixation for detecting adrenergic nerve structures containing neuromediators forming luminescent reaction products with glyoxylic acid, in which the material is incubated during 15-20 min at temperature of 20-25°C. Then it is dried and heated during 5-10 min in thermostat at 80°C, studied with luminescent microscope, subjected to morphometric study and photographed. Then, histochemical reaction is set on the same material for detecting cholinergic nerve structures.

EFFECT: high comparison accuracy on the same portion of tissue being used.

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