DIAGNOSTIC TECHNIQUE FOR NEAR-GRAFT INFECTION OF JOINTS Russian patent published in 2018 - IPC G01N33/50 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to traumatology and orthopedics and can be used to diagnose periprosthetic joint infection in endoprosthetics. Essence of method: conduct a microscopic examination of smear of aspirate, stained by Gram, and further measuring the concentration of D-lactate using the D-lactam test system, test sample is prepared by adding to the 25 mcl aspirate from the endoprosthetic joint 45 mcl of the enzyme mix and 80 mcl of the substrate mixta, turbidity control is obtained by adding 45 mcl of distilled water and 80 mcl of a substrate mix to 25 mcl of aspirate from the endoprosthetic joint; resulting mixtures are incubated in a thermostat at a temperature of 37 °C±1 °C for 30 minutes and measure the optical density of the mixtures on a plate reader using a 570–600 nm filter. Then optical density of each test sample is subtracted from difference in optical density of turbidity control and empty cell and for each test sample, the concentration is calculated from the calibration schedule and at a D-lactate value of 1.2 mmol/L and more diagnosed as periprosthetic joint infection.

EFFECT: highly accurate method for the diagnosis of periprosthetic joint infection has been proposed, which allows fast, within one hour, to identify the presence of infection in the endoprosthetic joint, caused even by hard-to-cultivate microorganisms, which will allow to widely use the claimed method in the departments of traumatology, orthopedics and endoprosthetics.

1 cl, 1 tbl, 1 ex