FIELD: medicine.
SUBSTANCE: carry out the collection of biological material and transport for research. The study includes primary microscopy, bacteriological study and the output of the result. As a biological material at the preoperative stage, the synovial fluid is taken before the initial endoprosthetics, aspiration from the joint cavity before the revision prosthetics. As a biological material, samples of periprosthetic tissue and extracted implants in the amount of 4-6 samples from various points, bone cement, adjacent to the implant are used. Synovial fluid or aspirate from the joint cavity is taken for examination both before the operation and during the operation. Perform the preparation of the material for cultivation and its sowing. All cultures are incubated at 35°C up to 14 days. In the absence of growth of microorganisms after 5 days, a preliminary result is given, and within 14 days - the final result.
EFFECT: use of the invention makes it possible to improve the quality of microbiological studies by isolating slow-growing and poorly cultivated microorganisms.
2 ex
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Authors
Dates
2017-08-08—Published
2015-11-13—Filed