METHOD FOR RENATURED PROTEIN G ISOLATION FROM MARKED POLYACRYLAMIDE GEL Russian patent published in 2018 - IPC C07K1/14 C07K1/26 

FIELD: biotechnology.

SUBSTANCE: method is proposed for renatured protein G isolation from the marked polyacrylamide gel. The proposed method includes the steps of marking of the protein G position in a polyacrylamide gel, separation of fragment of the marked gel containing protein G, sequential treatment of the gel fragment with a denaturing buffer solution containing 20 mM of Tris (pH 8.0), 100 mM of NaCl, 1% Triton X-100, 6 M urea, 2 M thiourea for 1 hour and a renaturing buffer solution containing 20 mM of Tris (pH 8.0), 100 mM of NaCl, 2 mM of sulfobetaine, 1 mM of EDTA, for 3 hours at 20°C with a three-fold change in the buffer and final electro-elution of the renatured protein G from the ground gel in a buffer solution containing 10 mM potassium phosphate buffer (pH 7.5), for 2 hours and at a current strength of 200 mA.

EFFECT: method can be used to isolate protein G, which has preserved immunobiological properties.

7 dwg, 7 ex