FIELD: biotechnology.
SUBSTANCE: inventions relate to the release of baculovirus-expressed virus-like particles (VLP) of non-enveloped viruses from insect cells and relate to a method for releasing baculovirus-expressed VLP of porcine circovirus type 2 (PCV2) in insect cells and a method for producing baculovirus-expressed VLP PCV2. Present method for releasing baculovirus-expressed VLP PCV2 comprises a step of mixing insect cells selected from SF9 and SF21 with a salt, where solution of salt in water, obtained as a result of mixing contains from 300 mOsmol/l to 600 mOsmol/l salt, selected from the group consisting of NaCl, KCl, CaCl2 and MgCl2, and does not include lysis or cell destruction steps.
EFFECT: inventions enable to obtain non-secreted VLP in a culture liquid without the need to isolate the entire cellular nucleic acid and protein content.
6 cl, 8 dwg, 1 tbl, 8 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT EXPRESSION OF PROTEIN ORF2 PCV2b IN INSECT CELLS |
2018 |
- Sondermeijer, Paulus, Jacobus, Antonius
- Sanders, Lisette
- Van Der Heijden Liefkens, Karin, Huberdina, Antonia
|
RU2779423C2 |
| METHOD FOR PREPARING A READY-TO-USE COMBINED PCV2 / MYCOPLASMA HYOPNEUMONIAE VACCINE |
2015 |
- Jansen, Theodorus
- Witvliet, Maarten, Hendrik
- Jayappa, Huchappa, Gowda
|
RU2731845C2 |
| IMMUNOGENIC COMPOSITIONS PCV2 AND METHODS FOR PRODUCING SUCH COMPOSITIONS |
2005 |
- Ehjchmejer Mark
- Nittsel' Greg
- Sheffer Merrill
|
RU2435854C2 |
| PCV2 IMMUNOGENIC COMPOSITIONS AND METHODS FOR PRODUCING THESE COMPOSITIONS |
2011 |
- Ehjchmejer Mark
- Nittsel' Greg
- Sheffer Merrill
|
RU2575615C2 |
| PCV2 IMMUNOGENIC COMPOSITIONS AND METHODS OF SUCH COMPOSITIONS PRODUCTION |
2005 |
- Ejchmejer Mark
- Nittsel Greg
- Sheffer Merrill
|
RU2620968C9 |
| PCV2B DIVERGENT VACCINE COMPOSITION AND METHODS OF USE |
2014 |
- Nitzel Gregory Paul
- Slade David Ewell
|
RU2662685C2 |
| RECOMBINANT VIRUS-LIKE CIRCOVIRUS CAPSID PARTICLE (VLP): COMPOSITIONS, METHODS AND USES |
2020 |
- Galarsa, Khose M.
- Ven, Ke
- Gavrilov, Boris
|
RU2817679C2 |
| RECOMBINANT PLASMID pFastBAC-B17R DNA CONTAINING COWPOX VIRUS GENOME FRAGMENT CODING ALPHA/BETA-INTERFERON BINDING PROTEIN AND BvB17RG BACULOVIRUS STRAIN PRODUCING SOLUBLE ALPHA/BETA-INTERFERON BINDING PROTEIN OF COWPOX VIRUS |
2009 |
- Gileva Irina Pavlovna
- Nepomnjashchikh Tat'Jana Sergeevna
- Rjazankin Igor' Aleksandrovich
- Shatrova Natal'Ja Mikhajlovna
- Shchelkunova Galina Aleksandrovna
- Shchelkunov Sergej Nikolaevich
|
RU2405824C1 |
| RECOMBINANT PLASMID DNA pFastBac-G2R-IgG, WHICH CONTAINS FRAGMENT OF SMALLPOX VIRUS GENOME, CODING FACTOR OF TUMOUR NECROSIS, BINDING PROTEIN, AND FRAGMENT OF HUMAN GENOME, CODING SECTION OF HEAVY CHAIN OF ANTIBODY G, AND STRAIN OF BACULOVIRUS BvG2RIgG, WHICH PRODUCES SOLUBLE CHIMERIC PROTEIN, WHICH CONSISTS OF SMALLPOX VIRUS PROTEIN, BINDING FACTOR OF TUMOUR NECROSIS, AND FRAGMENT OF HEAVY CHAIN OF HUMAN ANTIBODY G |
2008 |
- Nepomnjashchikh Tat'Jana Sergeevna
- Gileva Irina Pavlovna
- Lebedev Leonid Rudol'Fovich
- Shchelkunov Sergej Nikolaevich
|
RU2376375C2 |
| FUNCTIONAL INFLUENZA VIRUS-LIKE PARTICLES (VLPs) |
2006 |
- Smit Gejl
- Brajt Rik
- Pushko Piter
- Chzhan Tszin'Ju
- Makhmud Kutub
|
RU2483751C2 |
