RECOMBINANT EXPRESSION OF PROTEIN ORF2 PCV2b IN INSECT CELLS Russian patent published in 2022 - IPC C07K14/05 A61K39/12 A61K39/39 C12N5/07 C12N5/10 C12N15/34 C12N15/86 A61P31/20 

FIELD: biotechnology; veterinary medicine.

SUBSTANCE: invention relates to the field of biotechnology and veterinary medicine, in particular to an insect cell providing optimized expression of mutant protein ORF2 PCV2b; to a virus-like particle (VLP) of mutant protein ORF2 PCV2b; to a method for expression and production of mutant protein ORF2 PCV2b in insect cells; to a vaccine for pigs for suppression of PCV2 infection or disease signs related to it, containing mutant protein ORF2 PCV2b, and to its production method; to a method for suppression of PCV2 infection or disease signs related to it in a pig, using the above-mentioned vaccine, as well as to mutant protein ORF2 PCV2b, and its use.

EFFECT: mutation in protein ORF2 PCV2b allows for prevention of its accumulation in a nucleus and, thereby, optimization of its expression in insect cells.

14 cl, 4 dwg, 4 tbl, 3 ex