METHOD FOR DETERMINING SENSITIVITY OF MICROORGANISMS TO DISINFECTANTS Russian patent published in 2018 - IPC C12Q1/04 A61L2/16 

FIELD: microbiology.

SUBSTANCE: invention relates to microbiology and disinfectology and can be used to assess the sensitivity of microorganisms to disinfectants. Method involves applying, with a metering device, a suspension of the microorganisms being onto the surface of a Petri dish and drying the microorganism suspension. After drying, the infected surface of the dish is treated with a disinfectant by irrigation, wiping or immersion, and temporary exposure. Then a neutraliser solution corresponding to the chemical composition of the disinfectant used is added to the Petri dish, and after the aging time, agar melted and cooled to 45 °C is added. Dish is placed in a thermostat at optimum temperature and after aging, the number of colonies grown is counted. Based on the results of comparison with data obtained with a similarly contaminated cup, treated similarly to the experimental cup, but with sterile tap water, judgment is made about the sensitivity of the tested microorganisms to the disinfectant. Decrease in the number of microorganisms as a result of the disinfectant solution by 99.00–100 % indicates their sensitivity, and a decrease of less than 99.99 % indicates resistance.

EFFECT: invention improves accuracy of the determination method.

1 cl, 1 tbl, 1 ex