FIELD: biotechnology.
SUBSTANCE: method of scanning gene mutations in human tumours is described. It includes amplification of analyzed gene fragment, melting duplexes of TaqMan probes with single-stranded amplicons, building peak melting, identification of mutations by the asymmetry of the melting peaks, featured by double-stranded scanning gene mutations by symmetric real-time PCR with two TaqMan probes used for post-amplification of DNA melting.
EFFECT: method allows for the quantitative measurement of the number of copies of the analyzed gene in the original DNA sample, for simultaneous scanning of mutations in both amplicon threads, that improves reliability and sensitivity of the analysis, and provides additional information about the type of mutation.
6 dwg, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR IDENTIFICATION OF BRAF GENE MUTATIONS IN HUMAN TUMOURS | 2016 |
|
RU2631824C1 |
| METHOD OF ANALYZING TERMINAL MUTATIONS IN BRCA1, BRCA2, ATM AND PALB2 GENES USING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH AN OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2020 |
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| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN BRAF, NRAS AND KIT GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2017 |
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RU2674338C1 |
| REAGENT AND METHOD OF PREVENTING AT LEAST ONE FALSE START IN POLYMERASE CHAIN REACTION AMPLIFICATION, SETS OF REAGENTS AND OLIGONUCLEOTIDES FOR AMPLIFICATION BY POLYMERASE CHAIN REACTION | 2005 |
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RU2495046C2 |
| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN GNAQ AND GNA11 GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2017 |
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RU2674687C1 |
| NA HYBRIDISATION PROCEDURE | 2009 |
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RU2423522C2 |
| METHOD OF DETECTION OF DELETION IN DAT RAT LINE | 2023 |
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RU2811140C1 |
| METHOD OF ANALYSIS OF SOMATIC MUTATIONS IN GENES EGFR, KRAS AND BRAF USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2014 |
|
RU2552483C1 |
| METHOD OF DIAGNOSING MUCOPOLYSACCHARIDOSIS-PLUS SYNDROME | 2022 |
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RU2798695C1 |
| METHOD OF AMPLIFICATION AND DETECTION OF NUCLEOTIDE SEQUENCES IN REACTION MIXTURE AND SET USED THEREIN | 2009 |
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