FIELD: biotechnology.
SUBSTANCE: method comprises amplification of fragments of genes EGFR, KRAS and BRAF using LNA-blocking multiplex "nested" PCR in which the template for amplification is used as DNA tumour sample. The single-stranded fluorescently labelled PCR product is obtained. The biochip for identification of somatic mutations in the genes EGFR, KRAS and BRAF is obtained. Hybridisation of fluorescently labelled PCR-product in the biochip is carried out. The results of hybridisation on the biochip are recorded and interpreted.
EFFECT: invention enables to carry out analysis in a short period of time and with low material costs.
4 cl, 2 dwg, 4 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN PI3K GENE USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2013 |
|
RU2549682C1 |
| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN BRAF, NRAS AND KIT GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2017 |
|
RU2674338C1 |
| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN GNAQ AND GNA11 GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2017 |
|
RU2674687C1 |
| METHOD OF ANALYZING TERMINAL MUTATIONS IN BRCA1, BRCA2, ATM AND PALB2 GENES USING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH AN OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2020 |
|
RU2729360C1 |
| METHOD OF ANALYSIS OF SOMATIC MUTATIONS IN THE BCR/ABL CHIMERIC GENE USING RT-PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2015 |
|
RU2609641C1 |
| METHOD FOR DETERMINING PULMONARY TUMOUR SENSITIVITY TO TYROSINE KINASE INHIBITOR THERAPY | 2012 |
|
RU2499994C1 |
| METHOD FOR IDENTIFICATION OF BRAF GENE MUTATIONS IN HUMAN TUMOURS | 2016 |
|
RU2631824C1 |
| METHOD FOR IDENTIFICATION OF GENETIC POLYMORPHISMS AFFECTING METABOLISM OF ANTICANCER DRUGS USING BIOLOGICAL MICROCHIPS | 2018 |
|
RU2697096C1 |
| MOLECULAR GENETIC METHOD OF TUMOUR SENSITIVITY TEST IN PATIENTS WITH LUNG CANCER TO GEFITINIB THERAPY | 2010 |
|
RU2454464C2 |
| METHOD FOR DETERMINING GENETIC MARKERS FOR ASSESSING POLYGENIC RISK OF DEVELOPING HORMONE-POSITIVE SUBTYPE OF BREAST CANCER | 2020 |
|
RU2753002C1 |
