FIELD: biology; medicine.
SUBSTANCE: invention relates to the field of genetics, molecular biology and medicine. Method for detecting somatic mutations is proposed Q209P (s. 626A>C), Q209L (s. 626A>T), Q209R (s. 626A>G) in the gene GNAQ and Q209L (s. 626A>T), Q209P (s. 626A>C) in the gene GNA11. Carry out the amplification of fragments of the genes GNAQ and GNA11 using LNA-blocking multiplex "nest" PCR. Biochip is used to identify somatic mutations in the GNAQ and GNA11 genes and hybridization of labeled PCR product on the biochip. Record the results of hybridization.
EFFECT: invention provides the creation of an analysis method that allows to detect 5 somatic mutations in a short time and with little material costs.
3 cl, 2 dwg, 4 ex
| Title |
Year |
Author |
Number |
| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN BRAF, NRAS AND KIT GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) |
2017 |
- Emelyanova Marina Aleksandrovna
- Zasedatelev Aleksandr Sergeevich
- Nasedkina Tatyana Vasilevna
|
RU2674338C1 |
| METHOD OF ANALYZING TERMINAL MUTATIONS IN BRCA1, BRCA2, ATM AND PALB2 GENES USING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH AN OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) |
2020 |
- Tyulyandin Sergej Alekseevich
- Emelyanova Marina Aleksandrovna
- Pokataev Ilya Anatolevich
- Fesenko Denis Olegovich
- Abramov Ivan Sergeevich
- Khomich Darya Aleksandrovna
- Zasedatelev Aleksandr Sergeevich
|
RU2729360C1 |
| METHOD OF ANALYSIS OF SOMATIC MUTATIONS IN GENES EGFR, KRAS AND BRAF USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) |
2014 |
- Emel'Janova Marina Aleksandrovna
- Nasedkina Tat'Jana Vasil'Evna
- Ljubchenko Ljudmila Nikolaevna
- Zasedatelev Aleksandr Sergeevich
|
RU2552483C1 |
| METHOD OF ANALYSIS OF SOMATIC MUTATIONS IN THE BCR/ABL CHIMERIC GENE USING RT-PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) |
2015 |
- Ikonnikova Anna Yurevna
- Nasedkina Tatyana Vasilevna
- Zasedatelev Aleksandr Sergeevich
|
RU2609641C1 |
| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN PI3K GENE USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) |
2013 |
- Barskij Viktor Evgen'Evich
- Nasedkina Tat'Jana Vasil'Evna
- Emel'Janova Marina Aleksandrovna
- Abramov Ivan Sergeevich
- Pan'Kov Sergej Vasil'Evich
|
RU2549682C1 |
| SET OF SEQUENCES OF OLIGONUCLEOTIDES FOR DIAGNOSTICS OF GERMINAL MUTATIONS IN GENE RET, ASSOCIATED WITH GENETIC PREDISPOSITION TO CANCER OF THYROID |
2013 |
- Ljubchenko Ljudmila Nikolaevna
- Nasedkina Tat'Jana Vasil'Evna
- Amosenko Faina Arkad'Evna
- Abramov Ivan Sergeevich
- Filippova Margarita Gennad'Evna
|
RU2524433C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR GENETIC TYPING OF POLYMORPHOUS DNA LOCI ASSOCIATED WITH A RISK OF PROGRESSION OF SPORADIC FORM OF ALZHEIMER'S DISEASE IN RUSSIAN POPULATIONS |
2014 |
- Nasedkina Tatyana Vasilevna
- Gra Olga Alekseevna
- Nizamutdinov Igor Igorevich
- Zasedatelev Aleksandr Sergeevich
|
RU2600874C2 |
| METHOD OF ANALYZING POLYMORPHIC MARKERS IN THE GENES VKORC1, CYP4F2, CYP2C9, CYP2C19, ABCB1, ITGB3 TO DETERMINE INDIVIDUAL SENSITIVITY TO ANTICOAGULANT DRUGS |
2018 |
- Ikonnikova Anna Yurevna
- Nasedkina Tatyana Vasilevna
- Zasedatelev Aleksandr Sergeevich
|
RU2689400C1 |
| METHOD FOR DETERMINING GENETIC MARKERS FOR ASSESSING POLYGENIC RISK OF DEVELOPING HORMONE-POSITIVE SUBTYPE OF BREAST CANCER |
2020 |
- Ikonnikova Anna Yurevna
- Nasedkina Tatyana Vasilevna
|
RU2753002C1 |
| METHOD OF DETERMINING POLYMORPHIC MARKERS IN CYP2C19 AND CYP2D6 GENES FOR DETERMINING INDIVIDUAL SENSITIVITY TO ANTIDEPRESSANTS |
2018 |
- Ikonnikova Anna Yurevna
- Pozhitnova Viktoriya Olegovna
- Nasedkina Tatyana Vasilevna
- Zasedatelev Aleksandr Sergeevich
|
RU2716589C1 |
