FIELD: medicine; biotechnology.
SUBSTANCE: invention relates to molecular immunology, biotechnology and medicine, specifically to production of antibodies specifically blocking tumour-associated MUC1 and can be used in medicine. Recombinant single-chain antibody binding the tumour-associated MUC1, having a cytotoxic effect on the cancer cell line, and methods for its preparation using strains of Escherichia coli Rosetta2 (DE3) and yeast Pichia pastoris X33 and GS115.
EFFECT: technical result of the proposed invention is the reduced immunogenicity of the resulting antibody.
3 cl, 5 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| HUMANISED ANTIBODIES AND Fab BOUND WITH ANTIGEN F1 OF Yersinia pestis, AND METHOD FOR PRODUCING THEREOF WITH USING YEAST | 2009 |
|
RU2420587C2 |
| HUMANISED ANTIGEN-BINDING FRAGMENTS (Fab) AGAINST RABIES VIRUS, ISOLATED DNA FRAGMENT, CODING Fab AGAINST RABIES VIRUS, YEAST CELL, TRANSFORMED BY DNA FRAGMENT, AND METHOD OF OBTAINING Fab AGAINST RABIES VIRUS WITH APPLICATION OF YEASTS | 2010 |
|
RU2440412C2 |
| pVEAL2-9E2ch-SCFv PLASMID GENETIC CONSTRUCT, STRAIN OF RECOMBINANT CELL LINE CHO-K1-9E2ch AND CHIMERIC SINGLE-CHAIN ANTIBODY 9E2ch AGAINST WEST NILE VIRUS PRODUCED BY THE SPECIFIED STRAIN OF CELL LINE CHO-K1-9E2ch WITH HIGH AFFINITY FOR NEONATAL FcRn RECEPTOR | 2022 |
|
RU2801532C1 |
| RECOMBINANT YEAST TRANSFORMANT AND METHOD FOR OBTAINING WITH ITS USE OF Fc-FRAGMENT OF IMMUNOGLOBULIN | 2014 |
|
RU2664862C2 |
| PRODUCTION OF GLYCOPROTEINS WITH MODIFIED FUCOSYLATION | 2008 |
|
RU2479629C2 |
| HUMANISED ANTIBODY AND ANTIGENBINDING FRAGMENT (Fab), WHICH BINDS WITH HUMAN INTERFERON-γ, DNA FRAGMENTS CODING CLAIMED ANTIBODY AND ANTIGEN-BINDING FRAGMENT, CELL, TRANSFORMED BY DNA FRAGMENT, AND METHOD OF OBTAINING CLAIMED ANTIBODY AND ANTIGEN-BINDING FRAGMENT | 2013 |
|
RU2539752C2 |
| ANTIBODY SPECIFICALLY BINDING WITH MUC1 AND ITS APPLICATION | 2018 |
|
RU2746413C1 |
| RECOMBINANT Fab-scFv BASED ON A NEUTRALISING ANTIBODY AGAINST HUMAN BETA-1A INTERFERON AND AN ANTIBODY AGAINST HUMAN ErbB2 RECEPTOR | 2019 |
|
RU2748953C1 |
| PICHIA ANGUSTA RECOMBINANT YEAST STRAIN - PRODUCER OF CAPSID PROTEIN L1 OF HUMAN PAPILLOMAVIRUS TYPE 18 | 2011 |
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RU2445358C1 |
| ANTIBODY FOR BLyS | 2013 |
|
RU2613422C2 |
