RECOMBINANT YEAST TRANSFORMANT AND METHOD FOR OBTAINING WITH ITS USE OF Fc-FRAGMENT OF IMMUNOGLOBULIN Russian patent published in 2018 - IPC C12N15/64 C12N15/81 C12N1/19 C07K16/00 

FIELD: chemistry.

SUBSTANCE: group of inventions refers to bioengineering and biotechnology. Transformant of yeast Pichia sp. and a method for producing a human IgG4 Fc fragment. Transformant is modified by introducing an expression vector comprising a polynucleotide encoding the Fc fragment of the human IgG4 into the yeast Pichia sp., wherein the expression vector is pPIC9K-IgG4Fc, represented by the restriction map Fig. 1, or pPIC9K-mPSCFc, represented by the restriction map Fig. 2. To produce the Fc-fragment of IgG4, this transformant is cultured and immunoglobulin Fc fragment is isolated from the culture.

EFFECT: inventions make it possible to obtain the human Fc fragment of human IgG4 using a simple purification method that does not require an additional refolding process in isolating protein and additional amino acid residues at the N-terminus.

7 cl, 3 dwg, 3 ex