FIELD: medicine; biotechnology.
SUBSTANCE: invention relates to medicine, biology and biotechnology and is intended to determine the human genotype by polymorphism in the cytochrome gene P450 CYP2D6*6 (1707delT) rs5030655. Pair of primers is used, fluorescently labeled allele-specific oligonucleotide probes, and a universal oligonucleotide labeled with a fluorescence quencher are used. Assignment of a sample to a homozygote or heterozygote for a given allele is evaluated by the shape of the DNA melting curves, namely, by the maximum of the first derivative of the fluorescence plots.
EFFECT: invention allows to conduct a study in a single tube, which reduces the cost of the study.
1 cl, 1 dwg, 2 tbl
| Title | Year | Author | Number |
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