RAPID METHOD FOR BIOTESTING NATURAL AND WASTE WATER AND AQUEOUS SOLUTIONS Russian patent published in 2011 - IPC C12Q1/00 

FIELD: chemistry.

SUBSTANCE: rapid method involves preparation of working and control bioluminescent samples based on enzymes and measurement of bioluminescence kinetics. The control sample is prepared using a bioluminescent biomodule which contains co-immobilised enzymes and substrates of a bienzymatic system of luminescent bacteria with addition of distilled water or a phosphate buffer. The working sample is prepared using a bioluminescent biomodule which contains co-immobilised enzymes and substrates of a bienzymatic system of luminescent bacteria with addition of the analysed water sample. Further, both samples are held for 30-300 seconds; a bioluminescent reaction is initiated in both samples using an FMN solution, the volume ratio of the FMN solution in concentration of 5×10^-4 M to the control or analysed solution lying between 1:10 and 1:50; and bioluminescence intensity of both samples is measured. The water toxicity criterion is the 20% or more fall or 20% or more increase in the value of the maximum bioluminescence intensity measured in the working sample compared to these parameters in the control sample.

EFFECT: invention increases sensitivity of the rapid method for biotesting natural and waste water and aqueous solutions.

3 dwg, 6 ex