PLANT BIOTIC AGENT PHENOTYPING PLATFORM AND PROCESS OF PHENOTYPING Russian patent published in 2018 - IPC A01G31/02 A01G7/00 A01H17/00 

FIELD: biology.

SUBSTANCE: group of inventions relates to plant growing and biology. Plant biotic agent phenotyping platform comprises a container hermetically sealed with a lid delimiting an internal space divided into two spaces: lower internal space which comprises at least one temperature control means immersed in a temperature transfer liquid; and upper internal space, wherein the surface of the temperature transfer liquid is the border between the lower internal space and the upper internal space; lid comprising at least one orifice which is adapted to fit a pot and at least one pot whose bottom part is contained in the upper internal space and which is adapted to receive plant seeds and soil parasite preferably on soil, and to enable growth of the plant and development of such soil parasites. Kit comprises the phenotyping platform and at least one of irrigation means and/or lightning means for lightning the pot and/or decontamination means. Phenotyping greenhouse comprises one or more phenotyping platform and at least one irrigation means and/or lightning means for lightning the pot and/or decontamination means and/or means to control the temperature and hygrometry of the greenhouse. Method of identification of plant resistance or tolerance to a root parasite or interaction with a symbiotic organism comprises the steps of: sowing a plant seed on a substrate containing a standard quantity of soil parasite in at least one pot of a phenotyping platform; cultivating the plant; recovering the plants after a predetermined duration of cultivating; describing the roots and/or aerial damage on the plant. Method of identification of soil parasite population aggressiveness to a plant comprises the steps of: sowing plant seeds on a substrate containing different quantity of soil parasite of different origin in at least one pot of the phenotyping platform; cultivating the plant; recovering the plants after a predetermined duration of cultivating; describing the roots and/or aerial damage on the plant. Method of determination of the races of a soil parasite, especially Orobanche, comprises the steps of: placing in each pot of the platform a soil parasite and different plants for which the interaction with different soil parasite are known from the previous experiments; observing the plants behavior and determining the race of the soil parasite placed in the pot; optionally recovering DNA and/or RNA from the soil parasite, especially Orobanche, for genotyping. Method of identification of plant resistance or tolerance to drought comprises the steps of: sowing a plant seed on a substrate with a standard moisture in at least one pot of the phenotyping platform; cultivating the plant, according controlled drought conditions; recovering the plants after a predetermined duration of cultivating; describing the roots growth and structure of the plant.

EFFECT: inventions provide a rapid and reliable determination of the interaction between plants and various races of the soil parasite.

22 cl, 10 dwg, 1 tbl, 4 ex