METHODS, SYSTEMS, AND SOFTWARE FOR IDENTIFYING BIO-MOLECULES USING MODELS OF MULTIPLICATIVE FORM Russian patent published in 2018 - IPC G01N33/50 

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine and biotechnology, and can be used to conduct directed evolution of proteins. This includes (a) obtaining sequence and activity data for each of a variety of protein variants; (b) generating a multiplicative sequence-activity model from sequence and activity data, wherein the sequence-activity model binds the amino acids of the variant protein or nucleotides encoding the variant of the protein to the activity of the variant of the protein, and the multiplicative sequence-activity model comprises: product of multiple non-interacting multiplicative terms, each non-interacting multiplicative term contains the product of an independent variable and coefficient, where: (i) independent variable represents a particular amino acid of a variant of the protein or a specific nucleotide encoding a variant of the protein, and (ii) coefficient represents a contribution to the activity of the variant bean only by a specific amino acid or a specific nucleotide (c) use of a sequence-activity model to effect a cycle of directed evolution of one or more protein variants. Computer system for carrying out the directed evolution of proteins is also proposed.

EFFECT: group of inventions provides identification of biomolecules with the desired properties or most suitable for obtaining such properties from libraries of complex biomolecules or sets of such libraries.

32 cl, 9 dwg, 7 tbl