FIELD: biochemistry.
SUBSTANCE: invention relates to the field of biochemistry and genetic engineering, in particular to a constructed single guiding nucleic acid. This nucleic acid targets a nucleic acid and is capable of complexing with the Cas9 CRISPR II type protein. Present nucleic acid comprises a spacer sequence, a duplex, a linker sequence, and a tracr sequence, and is characterized in that it does not contain the region of the first stem. Invention also relates to a composition and to a kit that contain said nucleic acid.
EFFECT: present invention makes it possible to obtain a constructed single guiding nucleic acid targeting a nucleic acid and capable of complexing with the Cas9 CRISPR II type protein.
18 cl, 42 dwg, 8 tbl, 34 ex
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