METHOD FOR DETERMINING POSTTRANSPLANTATION CHIMERISM IN ANALYSIS OF POINT MUTATIONS OF BASE SUBSTITUTION IN GENES F2, F5, F7, F13, FGB, ITGA2, ITGB3, PAI-1 Russian patent published in 2018 - IPC G01N33/50 C12Q1/68 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to hematology, and is intended for determining post-transplant donor chimerism in the analysis of point mutations of base substitution in the genes of thrombophilia. To assess chimerism, single nucleotide polymorphisms of genes F2, F5, F7, F13, FGB, ITGA2, ITGB3, PAI-1 polymerase chain reaction in real time. If the allelic specificities of the donor are not found in the donor, the presence of chimerism is determined, and in the case of the determination of the allele in the donor, which is absent in the recipient, the absence of chimerism after the transplantation of allogeneic hematopoietic stem cells (HSC). Invention provides a method for estimating hematopoietic chimerism based on the analysis of informative alleles in genes F2, F5, F7, F13, FGB, ITGA2, ITGB3, PAI-1.

EFFECT: proposed method allows to determine the marker loci necessary for assessing the patient's condition after HSC transplantation.

1 cl, 3 tbl, 2 ex