J591 MINIBODIES AND CYS-DIABODIES FOR TARGETED DELIVERY OF HUMAN PROSTATE SPECIFIC MEMBRANE ANTIGEN (PSMA) AND METHODS FOR THEIR USE Russian patent published in 2018 - IPC A61K49/00 A61K39/395 

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry, in particular to a minibody for binding to a prostate-specific membrane agent (PSMA) comprising a scFv sequence that can bind to PSMA, said scFv comprising a variable heavy domain (VH) linked to a variable light domain (VL) by a linker sequence; an artificial hinge region and a human IgG CH3 sequence; and an isolated polynucleotide that encodes said minibody. Also disclosed is an anti-PSMA cys-diabody for use in the manufacture of a drug for cancer diagnosis. Invention also relates to a method for diagnosing cancer associated with the expression of PSMA in a subject, comprising administering an anti-PSMA minibody or an anti-PSMA cys-diabody. Also disclosed is a method of treating cancer associated with the expression of PSMA in a subject using said minibody or cys-diabody.

EFFECT: invention allows targeted delivery of human prostate-specific membrane antigen (PSMA) using high-efficiency minibodies and cys-diabodies, as well as the diagnosis and treatment of cancer associated with PSMA expression.

110 cl, 27 dwg, 6 ex