NEW LUCIFERASES AND METHODS OF THEIR USE Russian patent published in 2018 - IPC C12N9/02 C12N15/53 C12N15/63 C12N15/85 C12Q1/66 C12Q1/68 

FIELD: biochemistry.

SUBSTANCE: group of inventions relates to biochemistry. Nucleic acid encoding a luciferase or its functional fragment is proposed and selected from the group consisting of a nucleic acid encoding a protein, characterized by the amino acid sequence represented in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34, a nucleic acid encoding a protein having a sequence, which is at least 65 % identical to the amino acid sequence shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 or 34, and a nucleic acid that encodes a protein, which includes the consensus sequence SEQ ID NO: 35. Luciferase or its functional fragment encoded by the specified nucleic acid is also proposed. Expression cassette, a host cell, and a transgenic organism containing these nucleic acid molecules is also proposed. In addition, a kit for labeling organisms and a method for labeling cells and cell organelles is proposed.

EFFECT: group of inventions provides for the expansion of technical means in the application of bioluminescent systems.

26 cl, 10 dwg, 1 tbl, 14 ex