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(19)

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2 596 398

(13)

(51)

IPC

C07D309/32(2006-01-01)

C12N9/02(2006-01-01)

C12Q1/66(2006-01-01)

(21) (22)

Application

2015106305/10, 2015-02-25

(24)

Start date

2015-02-25

(22)

Actual filing date

2015-02-25

(45)

Published

2016-09-10

(72)

Inventor

Yampolskij Ilya ViktorovichPetushkov Valentin NikolaevichPurtov Konstantin ViktorovichRodionova Natalya SergeevnaBaranov Mikhail Sergeevich

(73)

Holder

Zakrytoe Aktsionernoe Obshshestvo

METHOD AND REAGENTS FOR DETECTION OF LUCIFERASE ACTIVITY Russian patent published in 2016 - IPC C07D309/32 C12N9/02 C12Q1/66

Abstract RU 2596398 C1

FIELD: biochemistry.

SUBSTANCE: group of inventions relates to biochemistry. Disclosed is a method of detecting luciferase in biological samples using 3-hydroxy hispidin or 3-hydroxy bisnoriangonin as luciferin. Proposed compound for implementing said method represents a 3-hydroxy hispidin. Besides, disclosed is a method of detecting luciferase in the presence of hispidin-3-hydroxylase in biological samples using hispidin or bisnoriangonin as luciferin precursor and NADP. Also disclosed is a reagents kit for implementing said method, including hispidin or bisnoriangonin and NADP.

EFFECT: group of inventions enables detecting luciferase fungi in biological samples and can be used in a wide spectrum of the bioluminescent analyses in vivo and in vitro.

4 cl, 19 dwg, 1 tbl, 5 ex

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Authors

Yampolskij Ilya Viktorovich

Petushkov Valentin Nikolaevich

Purtov Konstantin Viktorovich

Rodionova Natalya Sergeevna

Baranov Mikhail Sergeevich

Dates

2016-09-10—Published

2015-02-25—Filed