FIELD: biochemistry.
SUBSTANCE: group of inventions relates to biochemistry. Disclosed is a method of detecting luciferase in biological samples using 3-hydroxy hispidin or 3-hydroxy bisnoriangonin as luciferin. Proposed compound for implementing said method represents a 3-hydroxy hispidin. Besides, disclosed is a method of detecting luciferase in the presence of hispidin-3-hydroxylase in biological samples using hispidin or bisnoriangonin as luciferin precursor and NADP. Also disclosed is a reagents kit for implementing said method, including hispidin or bisnoriangonin and NADP.
EFFECT: group of inventions enables detecting luciferase fungi in biological samples and can be used in a wide spectrum of the bioluminescent analyses in vivo and in vitro.
4 cl, 19 dwg, 1 tbl, 5 ex
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