FIELD: medicine; pharmacology.
SUBSTANCE: it is obtained a chimerous photo protein (photin), presented by amino-acid sequence of obeline protein, which part (from the rest in position 50 to the rest in position 94) is replaced by a homologous site of amino-acid sequence of clitine protein (the rests 53-97). The method of obtaining new chimerous photo protein by the method of recombinant DNA and vectors applied to it and cells is described. It is offered to use photo protein under the invention as the calcium indicator in various test systems in vitro and in vivo.
EFFECT: increased level of bioluminescence in comparison with natural protein.
11 cl, 6 dwg, 2 tbl, 3 ex
| Title |
Year |
Author |
Number |
| ENHANCED BIOLUMINESCENCE PHOTOPROTEINS AND APPLICATION THEREOF AS INTRACELLULAR CALCIUM INDICATORS |
2006 |
- Mastrojanni Nadja
- Kajnarka Sil'Vija
- Koratstsa Sabrina
|
RU2407750C2 |
| MUTANT PHOTOPROTEIN (VERSIONS) FOR DETERMINING INTRACELLULAR CALCIUM SIMULTANEOUSLY IN DIFFERENT CELL ORGANELLES |
2008 |
- Malikova Natal'Ja Petrovna
- Vysotskij Evgenij Stepanovich
- Markova Svetlana Vladimirovna
- Stepanjuk Galina Anatol'Evna
|
RU2402566C2 |
| METHODS FOR IDENTIFYING MODULATORS OF GPR92 |
2017 |
- Mcgrane, Scott, Joseph
- Gibbs, Matthew, Ronald
- Fine, Richard, Masten
- Klebansky, Boris
|
RU2742608C2 |
| RECOMBINANT PLASMID DNA pET19b-SurvOL, WHICH PROVIDES SYNTHESIS OF A HYBRID PROTEIN SURVIVIN-OBELIN (SurvOL) AND A HYBRID PROTEIN BOUND BY ANTI-SURVIVIN ANTIBODIES AND POSSESSING BIOLUMINESCENT ACTIVITY |
2021 |
- Bashmakova Evgeniia Evgenevna
- Panamarev Nikita Sergeevich
- Frank Liudmila Alekseevna
|
RU2770490C1 |
| COMPOSITION OF COMPLEMENTING FRAGMENTS OF MOLECULE OF PHOTOPROTEINS FROM HYDROZOA AND METHOD OF ITS APPLICATION FOR ANALYSIS OF INTERMOLECULAR INTERACTIONS |
2009 |
- Markova Svetlana Vladimirovna
- Vysotskij Evgenij Stepanovich
- Natashin Pavel Viktorovich
|
RU2418859C1 |
| METHOD AND REAGENTS FOR DETECTION OF LUCIFERASE ACTIVITY |
2015 |
- Yampolskij Ilya Viktorovich
- Petushkov Valentin Nikolaevich
- Purtov Konstantin Viktorovich
- Rodionova Natalya Sergeevna
- Baranov Mikhail Sergeevich
|
RU2596398C1 |
| NEW LUCIFERASES AND METHODS OF THEIR USE |
2017 |
- Yampolskij Ilya Viktorovich
|
RU2674894C2 |
| METHOD AND REAGENTS FOR DETECTING LUCIFERASE ACTIVITY |
2019 |
- Iampolskii Ilia Viktorovich
|
RU2744869C2 |
| EXTRACT FROM VEGETABLE SOMATIC EMBRYOS, METHOD FOR PREPARING AND APPLYING EXTRACT, DIAGNOSTIC SCHEME BASED ON EXTRACT AND ITS APPLYING |
2003 |
- Dolgov S.V.
- Shalojko L.A.
- Miroshnichenko D.N.
|
RU2261278C2 |
| FLUORESCENT METHOD FOR DETERMINING CALCIUM CONCENTRATION ON BASIS OF DISCHARGED PHOTOPROTEINS |
2009 |
- Kudrjasheva Nadezhda Stepanovna
- Belogurova Nadezhda Valentinovna
|
RU2411000C1 |
