METHOD FOR DETECTING SEQUENCES OF NUCLEOTIDES BY POLYMERASE CHAIN REACTION METHOD IN REAL TIME WITH UNIVERSAL PROBE Russian patent published in 2018 - IPC G01N33/52 C12Q1/686 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, in particular to a method for detecting a sequence of nucleotides when conducting a polymerase chain reaction. Disclosed is a method for detecting specific nucleotide sequences by real-time PCR, which includes preparation of the reaction mixture, at the same time at its 3' end the direct primer incorporates a region, complementary to the detectable nucleotide sequence, the next region, having a sequence identical to the fluorescent probe, the region near 5' end, which is two sequences complementary to each other, which are separated by a non-complementary region and hybridizing during the reaction, whereby the amplicon, obtained by using the reverse primer from the matrix containing the direct primer, receives the region for annealing the fluorescent probe, as well as two sequences complementary already at 3' end, one of which, after hybridization, acts as a primer and starts amplification with probe hydrolysis, completing the sequence on the amplicon to form the reverse primer annealing region on its 3' end, which allows this primer to start the amplification with hydrolysis of fluorescent probes annealed on the amplicon in subsequent cycles, which is accompanied by an increase in fluorescence, and using its assessment the presence of specific nucleotide sequences is detected.

EFFECT: invention allows the use of the same probe for the detection of any nucleotide sequences.

3 cl, 7 dwg, 3 ex