FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to compositions and methods for testing the activity of nicking endonuclease and polymerase in a reaction, including the use of substrate in the form of a nucleic acid molecule that detects the activity of nicking endonuclease and polymerase by releasing detected reporter (for example, fluorophore).
EFFECT: invention provides compositions and methods for detecting the activity of
nicking endonuclease and polymerase.
39 cl, 11 dwg, 1 tbl, 4 ex
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