HUMAN UTERINE CERVICAL STEM CELL POPULATION AND USES THEREOF Russian patent published in 2019 - IPC C12N5/71 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of cell biology and biotechnology, in particular to a method for isolating mesenchymal stem cells of the cervix, which are not tumor cells, and express cellular markers CD29, CD44, CD73, CD105 and CD90 and do not express cellular markers CD117, CD133, HLA-DR, TRA-81, CD45, CD34 and CD31, the use of isolated cervical tissue and the method for obtaining the conditioned medium. Said method includes the preparation of a cell suspension from uterine cervix tissue, obtained by desquamation of the cervix, the recovery of cells from the specified cell suspension, the incubation of these cells in a suitable cell culture medium and under conditions that allow cell proliferation and the selection of mesenchymal stem cells, where these cervical mesenchymal stem cells express cellular markers CD29, CD44, CD73, CD105 and CD90 and do not express cellular markers CD117, CD133, HLA-DR, TRA1-81, CD45, CD34 and CD31. It is followed by the incubation of these cells in the medium for cultivation and the recovery of cells from the medium.

EFFECT: invention makes it possible to isolate non-tumor mesenchymal stem cells of the cervix that express cellular markers CD29, CD44, CD73, CD105 and CD90 and do not express cellular markers CD117, CD133, HLA-DR, TRA-81, CD45, CD34 and CD31.

8 cl, 18 dwg, 4 tbl, 6 ex