FIELD: biotechnology; medicine.
SUBSTANCE: invention relates to biotechnology and medicine, in particular to methods of producing T-cells for T-cell therapy, comprising reducing a cell population characterized by a predetermined cell density, into contact with a nanomatrix based on anti-CD3/CD28 antibodies, characterized by a certain concentration, and culturing the cells, thereby obtaining a population of T-cells containing an increased percentage of at least one subtype of T-cells. In some embodiments, the method leads to an increase in the percentage of memory stem T-cells.
EFFECT: invention enables prolonging T-cell persistence in vivo for use in T-cell therapy.
23 cl, 18 dwg, 1 tbl, 2 ex
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